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Synopsis Being teachers we generally feel dearth of book in Biophysical chemistry which could provide most of the informations an undergraduate post graduate student of Biochemistry, Microbiology and other science discipline should know. Friends also shared the same idea. In most books of biophysical chemistry, authors deal with certain topics of their interests in great depth while leave other topics which are not of their interest, though of their similar importance, if not more, to the students. So being constantly insisted and hard pressed by beloved colleges and students. We ultimately ventured to write a book on Biophysical chemistry, which should be completely exam-oriented and research-oriented also. Our intention has been to write in a clear, lucid manner and readily understandable way in our style keeping always a vigilant watch to the student’s interest. This book mainly deals with forces which are physicochemical in nature and of importance to different biological systems and the utilizations of these forces in recent analytical techniques. In general, we believe the book should prove useful to undergraduate and most graduate students of Microbiology, Biochemistry, Medical sciences and pharmaceutical science, and other discipline of life sciences.