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Experimental Biotechnology: A Laboratory Manual

Sunita Dutta (Author) Abhijit Dutta (Author) Ashok Kumar Choudhary (Author) Practical Manual Series

The book is subdivided into seven sections this encompass: general procedures, like methods of pipetting, solution preparation, buffers and principles of common analytical instruments essential for laboratory biotechnology experiments.

The book also includes working with nucleic acid, bacteria, enzymes, proteins; cloning experiments and a few protocols on plant biotechnology. Emphasis have been given on DNA/RNA isolation from various sources, use of restriction enzymes, ligation techniques, cloning protocols, screening of transformed cells, various electrophoresis techniques, PCR protocol, etc.

The appendices in the last part are included to provide information important to he study of the above-mentioned practical as a whole.

The book will be useful to students belonging to Biotechnology, agriculture and allied fields. The idea behind this practical manual was thus to provide theoretical basis of the practical study items to be undertaken in the laboratory in a lucid manner.

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About the authors

Sunita Dutta

Sunita Dutta is a Reader in Zoology at Women’s College Ranchi. She acquired her bachelors, masters and Ph.D. degrees from Ranchi University. Her research focuses mainly on the impact of human habitation on the ground water regime and other causative factors that alters its physiochemical quality. She teaches undergraduate and postgraduate courses in environmental science, molecular biology, Biotechnology. She has under her credit several published research papers and has written a few chapters in encironment related book.

Abhijit Dutta

Abhijit Dutta is a Reader of Zoology at the University of Ranchi and Serves as a faculty member of the Environmental Science and Biotechnology programmes. He received bachelors and masters degree in Zoology from Ranchi University. He later acquired a Ph.D. in Environmental Science from the same university. He directs a laboratory of postgraduate students who work with him on toxicology and other environment related subjects. Presently seven research scholars are working under him, mainly on Cytotoxicity assay of several synthetic and biological toxins and their bioremediation. He has published several research papers and wrote many book chapters. He is the recipient of ZSI Gold Medal and is the life member of many professional bodies.

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Bibliographic information

Title Experimental Biotechnology: A Laboratory Manual
Format Hardcover
Date published: 10.02.2011
Edition 1st ed.
Language: English
isbn 9789380235721
length xii+254p., Illustrations; 25cm.