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Molecular Markers & Plant Biotechnology

Rukam S. Tomar (Author)

The book entitled `Molecular Markers and Plant Biotechnology' is an exclusive collection of molecular marker based techniques narrated in 40 chapters through 578 pages along with figures makes it essential for biotechnology people. To supplement the practical working the relevant equipments have been described. Laboratory safety rules placed in the beginning is a wise task. Appendices include basic calculations, basic principles in preparation of reagents, abbreviations and glossary show the carefulness while preparing this text. This is an unavoidable text for biotechnology laboratory and class.


1. Milestones in DNA History and Biotechnology.; 2. Equipments Required in a Molecular Marker Laboratory.; 3. Isolation, Purification and Quantification of Genomic DNA from Plants.; 4. Isolation, Purification and Quantification of RNA from Plants.; 5. Electrophoresis.; 6. Molecular Weight Markers for Gel Electrophoresis.; 7. Polyacrylamide Gel Electrophoresis (PAGE).; 8. Gel Electrophoresis of Protein.; 9. Isoenzyme.; 10. Extraction of DNA Fragments from Agarose Gel.; 11. Why Molecular Markers?.; 12. Restriction Enzyme Digestion of DNA and its Agarose Gel Electrophoresis.; 13. Thermocyclers (PCR Machines).; 14. Modifications of Basic PCR Technique.; 15. Random Amplified Polymorphic: A (RAPD).; 16. Restriction Fragment Length Polymorphism (RFLP).; 17. Amplified Fragment Length Polymorphism (AFLP).; 18. Simple Sequence Repeats (Microsatellite).; 19. Variable Number of Tandem Repeats (Minisatellite).; 20. Inter Simple Sequence Repeat (ISSR).; 21. Sequence Characterized Amplified Region (SCAR).; 22. Cleaved Amplified Polymorphic Sequence (CAPS).; 23. Single-Strand Conformation Polymorphism (SSCP).; 24. Retrotransposon-Based Markers (S-SAP, IRAP, REMAP, RBIP, RGAS, SNP).; 25. Silver Staining.; 26. Basics of Marker Assisted Selection in Crop Plants.; 27. Mapping Populations.; 28. QTL Mapping.; 29. Southern Blotting.; 30. Western Blotting.; 31. Northern Blotting.; 32. Eastern Blotting.; 33. DNA sequencing and designing of primers.; 34. Gene Transfer to Plant.; 35. Fluorescent in Situ Hybridization (FISH).; 36. Genotypic Barcoding.; 37. mtDNA.; 38. MicroRNA.; 39. Graphical Approach to Calculate Molecular Weights of DNA / Protein Fragments.; 40. Calculation of Similarity Index Values and Construction of Dendrogram Using NTSYSpc 2.0.; Appendices.; References.; Index.

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About the author

Rukam S. Tomar

Dr. Rukam S. Tomar has been working as Assistant Professor in Biotechnology at Junagadh Agricultural University.  He has done his Ph. D. in Plant Breeding and Genetics.  He has published 20 research papers in different National and International Journals and has also contributed two book chapters.

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Bibliographic information

Title Molecular Markers & Plant Biotechnology
Format Hardcover
Date published: 01.01.2010
Edition 1st ed.
Language: English
isbn 9380235259, 9789380235257
length xlviii+577p., Illustrations; 25cm.