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Synopsis The term 'Tissue culture' can be applied to any multicellular culture growing on a solid medium that consists of many cells in protoplasmic continuity. The culture of an explant, consisting of one or more tissue, results in a callus that has no structural or functional counterpart with any tissue of the normal plant body. The use of tissue culture methodologies for studies has a short but productive history in agronomy and horticulture. The very recent applications of these procedures for forest trees show tremendous potential to circumvent some of the difficult logistical problems of studying diseases in vivo. Technical and informational gains made in other plant sciences are now being examined by forest pathologists to supplement and accelerate traditional breeding programs. This book is aimed at all those interested and involved in the results of plants on the environment and is an essential basic reference book for students, academicians and specialists.