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1. Spectrophotometer 
2. Calibrating Spectrophotometer
3. Sample Collection and Precuations for Optimal Sampling
4. Estimation of Chloroplastic Pigment Contents
5. Hill Activity
6. Estimation of Carbohydrate Contents
7. Sugar Metabolizing Enzymes
8. Estimation of Oxidative Stress Markers
9. Antioxidant Enzymes
10. Assay of Glutathione Metabolizing Enzymes (Ascorbate-Glutathione Cycle)
11. Amino Acid Content
12. Carbonyl Content Estimation 
13. Assay of Krebs Cycle Intermediates
14. Extraction and Assay of Krebs Cycle Enzymes
15. Extraction and Assay of Phosphorolytic Enzymes
16. Extraction and Assay of GABA Synthesizing Enzymes and Estimation of GABA Contents
17. Nitrate Reductase (NR) Activity
18. Relative Water Content (RWC)
19. Membrane Stability Index (Sairam, 1994)
20. Protein Content (Lowry et al., 1951)
21. α-Amylase Activity
22. Amylose Content
23. Amylopectin
24. Chlorophyll stability index (CSI)
25. Gas Exchange Parameters
26. Biomass Accumulation and Allocation Pattern
27. Germination Parameters
28. Quantitative Drought Tolerant Parameters