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This second edition is thoroughly revised, updated and enlarged, for the readers. In this second edition, six new chapters have been added. These chapters are: (i) Bone marrow trephine biopsy, (ii) Tru-cut biopsy or Core needle biopsy, (iii) Diagnostic use of immunhistochemistry in surgical pathology, (iv) Microarrays and DNA sequencing, (v) Microdissection and different types of polymerase chain reaction, and (vi) Quality control in histopathology. The chapter on Frozen Section has been fully revised and more practical information have been included, keeping in mind the problems faced by the users during cryostat/frozen sections. Molecular diagnostic changes at a glance in different diseases, tumors and hereditary diseases have been added in the Appendix in Tabular form (almost like a new chapter!) and hope it will be useful for different examinations as last minute revision. Most of these new chapters have been added in this Second Edition as per requests by the readers, especially by the postgraduate students who are pursuing MD in Pathology. Key Features: The latest edition of Manual of Histological Techniques is a compilation of the basics and latest advances in this rapidly evolving field of histopathology . The exciting growth of medical technology providing new methods of investigation and treatment is very well imbibed in the essence of this book. All chapters are thoroughly revised with addition of 6 new chapters on bone marrow trephine biopsy, tru-cut biopsy, immunohistochemistry in surgical pathology, microarrays and DNA sequencing, microdissection and different types of PCR and quality control in histopathology. The chapter on frozen section has been thoroughly revised and expanded with problem solving tips faced during day-to-day practice. An appendix on molecular diagnostic changes at a glance in different diseases, tumors and hereditary diseases has also been included as a ready reckoner for the students and specialists working with surgical pathology laboratories. Written by an expert in the field the book is a ready reference for pathologists, postgraduate students and technicians working in the histopathological laboratories. 

Contents: 1. Tissue Fixation and Fixatives. 2. Tissue Processing, Microtomy and Paraffin Sections. 3. Cryostat and Frozen Section. 4. Staining of Tissues—Basic Concept. 5. Routine Hematoxylin and Eosin Stain. 6. Carbohydrates. 7. Lipids. 8. Proteins and Nucleic Acid Staining. 9. Connective Tissue Staining. 10. Pigments and Minerals. 11. Bone and Decalcification. 12. Nervous System. 13. Some Special Stains. 14. Enzyme Histochemistry and Histochemical Stains. 15. Immunohistochemistry. 16. Cell Block and Diagnostic Cytopathology. 17. Plastic/Resin Embedding Media. 18. Electron Microscopy. 19. Immunofluorescence Techniques. 20. Museum Technique. 21. Light Microscopy. 22. Molecular Diagnostic Techniques and Its Applications. 23. In Situ Hybridization and Fluorescent In Situ Hybridization. 24. Bone Marrow Trephine Biopsy. 25. Tru-cut Biopsy or Core Needle Biopsy. 26. Diagnostic Use of Immunohistochemistry in Surgical Pathology. 27. Microarrays and DNA Sequencing. 28. Microdissection and Different Types of Polymerase Chain Reaction. 29. Quality Control in Histopathology. Appendices. Glossary. Index.