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This treatise is an introductory book for fresh students entering into the field of microbiology. The fundamental techniques, which are basic to all laboratories involved in microbiological and associated works, have been described with illustrations. Moreover, concise information about different microorganisms such as bacteria, viruses, protozoa, microscopic fungi and microscopic algae has been given so as to acquaint the students with these microbes before starting any experiment on them. A total of 55 experiments have been described in a stepwise manner along with illustrative flow diagrams for all the experiments. All attempts have been made to make the manual user friendly by making each experiment a separate and independent one, so that it can be conducted without borrowing steps from any other experiment. A total of 128 illustrations and 27 illustrated reactions have made the manual a real illustrated one making its use very and simple. The book shall be a valuable piece of information and an easily comprehensible aid in microbiology laboratories for students, teachers, scientists, laboratory personnel and all associated with microbiology and allied subjects.